Title: Species-specific primers designed from RAPD products for Bithynia funiculata, the first intermediate host of liver fluke, Opisthorchis viverrini, in north Thailand
Online: http:/­/­dspace.­library.­tu.­ac.­th…
Creator: Jutharat Kulsantiwong
Sattrachai Prasopdee
Supawadee Piratae
Panita Khampoosa
Apiporn Suwannatrai
Wipada Duangprompo
Thidarut Boonmars
Wipaporn Ruangjirachuporn
Jiraporn Ruangsittichai
Vithoon Viyanant
Hebert, Paul D. N.
Smarn Tesana
Type: Article
Languge: en
Date: 2556-10-08T08:51:14Z
2013
Description: Bithynia funiculata is the first intermediate host of the human liver fluke Opisthorchis viverrini in northern Thailand but its identification through morphological analysis is often problematic due to the shortage of gastropod taxonomists. As a consequence, we focused on the development of species-specific primers for use as an identification tool. Our work involved recovery of a 502-base pair (bp) amplicon of unknown function through species-specific primers whose effectiveness was tested by analyzing specimens of B. funiculata from 3 locations in northern Thailand. This primer set did not amplify other species in the Bithyniidae or in other gastropod families. By providing a tool to confirm morphological identifications of B. funiculata, and by enabling the identification of juvenile specimens and those with damaged shells, these primers will improve estimates of the prevalence of parasitic infections in this snail. © 2013 American Society of Parasitologists.
Subject: Estimation method
Bithynia
Funiculata
DNA extraction
Thailand
Identifier: http://dspace.library.tu.ac.th/handle/3517/6713
Source: Thammasat Digital Library

Similar Items:

1
Species-specific primers designed from RAPD products for Bithynia funiculata, the first intermediate host of liver fluke, Opisthorchis viverrini, in north &hellip by Jutharat Kulsantiwong — Sattrachai Prasopdee — Supawadee Piratae — Panita Khampoosa — Apiporn &hellip
2
DNA barcode identification of freshwater snails in the family bithyniidae from Thailand by Jutharat Kulsantiwong — Sattrachai Prasopdee — Jiraporn Ruangsittichai — Wipaporn Ruangjirachuporn &hellip
3
DNA barcode identification of freshwater snails in the family bithyniidae from Thailand by Jutharat Kulsantiwong — Sattrachai Prasopdee — Jiraporn Ruangsittichai — Wipaporn Ruangjirachuporn &hellip
4
Temperature dependence of opisthorchis viverrini infection in first intermediate host snail, Bithynia siamensis goniomphalos by Sattrachai Prasopdee — Jutharat Kulsantiwong — Supawadee Piratae — Panita Khampoosa — Chalida &hellip
5
Temperature dependence of opisthorchis viverrini infection in first intermediate host snail, Bithynia siamensis goniomphalos by Sattrachai Prasopdee — Jutharat Kulsantiwong — Supawadee Piratae — Panita Khampoosa — Chalida &hellip
6
Effects of Bayluscide on Bithynia siamensis goniomphalos, the first intermediate host of the human liver fluke, Opisthorchis viverrini, in laboratory and field &hellip by Smarn Tesana — P. Thapsripair — Chalida Thammasiri — Sattrachai Prasopdee — Apiporn Suwannatrai &hellip
7
Effects of Bayluscide on Bithynia siamensis goniomphalos, the first intermediate host of the human liver fluke, Opisthorchis viverrini, in laboratory and field &hellip by Smarn Tesana — P. Thapsripair — Chalida Thammasiri — Sattrachai Prasopdee — Apiporn Suwannatrai &hellip
8
Light and electron microscopy observations of embryogenesis and egg development in the human liver fluke, Opisthorchis viverrini (Platyhelminthes, Digenea) by Panita Khampoosa — Jones, Malcolm K. — Lovas, Erica M. — Tuanchai Srisawangwong — Thewarach Laha &hellip
9
Light and electron microscopy observations of embryogenesis and egg development in the human liver fluke, Opisthorchis viverrini (Platyhelminthes, Digenea) by Panita Khampoosa — Jones, Malcolm K. — Lovas, Erica M. — Tuanchai Srisawangwong — Thewarach Laha &hellip
10
Opisthorchis viverrini : identification of a glycine-tyrosine rich eggshell protein and its potential as a diagnostic tool for human opisthorchiasis by Jiraporn Ruangsittichai — Vithoon Viyanant — Suksiri Vichasri-Grams — Prasert Sobhon — Smarn &hellip
11
Opisthorchis viverrini : identification of a glycine-tyrosine rich eggshell protein and its potential as a diagnostic tool for human opisthorchiasis by Jiraporn Ruangsittichai — Vithoon Viyanant — Suksiri Vichasri-Grams — Prasert Sobhon — Smarn &hellip
12
Veerachai Eursitthichai, Banchob Sripa, Sirikanda Thanasuwan, Thewarach Laha by Supawadee Piratae — Smarn Tesana — Jones, Malcolm K. — Brindley, Paul James — Loukas, Alex C. &hellip